A Two-Step Method for Obtaining Highly Pure Cas9 Nuclease for Genome Editing, Biophysical, and Structural Studies
MPs | Free Full-Text | A Two-Step Method for Obtaining Highly Pure Cas9 Nuclease for Genome Editing, Biophysical, and Structural Studies
A detailed protocol for expression, purification, and activity determination of recombinant SaCas9 - ScienceDirect
Purification of Cas9 protein. (A) SDS-PAGE (sodium dodecyl sulfate... | Download Scientific Diagram
Structural insights into the human PA28–20S proteasome enabled by efficient tagging and purification of endogenous proteins | PNAS
The Cas9-gRNA ribonucleoprotein complex-mediated editing of pyrG in Ganoderma lucidum and unexpected insertion of contaminated DNA fragments | Scientific Reports
Purification of Cas9 protein. (A) SDS-PAGE (sodium dodecyl sulfate... | Download Scientific Diagram
STANDARD OPERATING PROCEDURE DETAILS
Improving the efficiency of CRISPR-Cas9: The expression & purification of Cas9
MPs | Free Full-Text | A Robust Expression and Purification Method for Production of SpCas9-GFP-MBP Fusion Protein for In Vitro Applications
PURedit™ CRISPR Synthetic Guide RNAs and Cas9 Protein
Co-expression of Cas9 and single-guided RNAs in Escherichia coli streamlines production of Cas9 ribonucleoproteins | Communications Biology
Purification of Cas9 recombinant protein. (A) Purification of Cas9 by... | Download Scientific Diagram
Cas9 Proteins • NUCLEUS BIOTECH
A Expression of the recombinant Cas9-GFP protein, B purification of... | Download Scientific Diagram
Isolating Genomic Regions of Interest with the CRISPR System
Production of Purified CasRNPs for Efficacious Genome Editing - Lingeman - 2017 - Current Protocols in Molecular Biology - Wiley Online Library
Addgene: CRISPR Plasmids - Purify Genomic Loci
An efficient and cost-effective purification protocol for Staphylococcus aureus Cas9 nuclease - ScienceDirect
Physicochemical and Functional Characterization of Differential CRISPR-Cas9 Ribonucleoprotein Complexes | Analytical Chemistry
Improving the efficiency of precise genome editing with site-specific Cas9-oligonucleotide conjugates | Science Advances
Enhanced genome editing efficiency of CRISPR PLUS: Cas9 chimeric fusion proteins | Scientific Reports
